A Mechanism for Penicillinase Secretion in Bacillus licheniformis
نویسندگان
چکیده
منابع مشابه
Further evidence for a partially folded intermediate in penicillinase secretion by Bacillus licheniformis.
Protoplasis of Bacillus licheniformis 749/C (a mutant constitutive for penicillinase production) continued to synthesize and release penicillinase in hypertonic growth medium in the presence of trypsin and chymotrypsin at 25 mug each per ml. When the protoplasts were stripped of about half of their membrane-bound penicillinase by pretreatment at pH 9.5 or with a higher level of trypsin, penicil...
متن کاملA mechanism for penicillinasesecretion in Bacillus licheniformis.
Cell-bound isozymes of penicillinase are distinguished from extracellular enzyme by their capacity to bind deoxycholate and to elute with an apparent molecular weight of 45,000 on gel filtration in its presence. By methods that are unlikely to involve changes in primary structure, the cell-bound forms (both from the plasma membrane and from the periplasmic vesicles) can be converted to forms th...
متن کاملMorphological phenomena associated with penicillinase induction and secretion in Bacillus licheniformis.
Cells of uninduced Bacillus licheniformis (strain 749) in mid-logarithmic phase have no extensive intracytoplasmic membrane. After induction with cephalosporin C, characteristic organelles that contain tubules and vesicles with single-layered membranes and no visible internal substructure can be seen in thin sections in the periplasm. A magnoconstitutive penicillinase producer (749/C) contains ...
متن کاملImmunoelectron microscopic localization of penicillinase in Bacillus licheniformis.
Penicillinase was localized in log-phase cells of Bacillus licheniformis 749/C by labeling with ferritin-anti-penicillinase immunoglobulin G conjugate. Mildly fixed homogenized cells, isolated subcellular fractions, and frozen thin sections were labeled. The label was distributed in discrete patches in the cell envelope. The patches extended from the inside part of the membrane to the outside p...
متن کاملPhospholipid metabolism during penicillinase production in Bacillus licheniformis.
During membrane-bound penicillinase production, Bacillus licheniformis forms vesicles and tubules that do not appear in the absence of penicillinase production. The major lipids of B. licheniformis were shown to be phospholipids. The proportions, metabolism, and the total phospholipid per cell were shown to be essentially the same in the uninduced control, induced and constitutive penicillinase...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1970
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.65.4.962